Product datasheet

Summary

Product Identification

Millipore Ultrafiltration (UF) membranes are designed for macromolecule processing, separating particles and dissolved molecules ranging from 1,000 to 1,000,000 molecular weight. These void-free membranes are manufactured in a Class 100K controlled environment, adhering to GMP practices and ISO compliant measures.

Product families include:

• BioMax PB Membranes: Made of polyethersulfone (PES), these membranes offer high flux, low to moderate protein binding, and resistance to caustic and chlorine. They are modified to reduce non-specific protein binding and fouling. Suitable for protein solutions containing more than 0.1 mg/mL of protein.
  • Features: Composite PES membrane for stable hydraulics and mechanical strength, void-free structure for high flux, excellent retention, and higher yields.
• Ultracel PLC Membranes: Constructed from regenerated cellulose cast onto a microporous polyethylene substrate, these void-free membranes provide ultra-low protein binding, low fouling, solvent resistance, and improved back pressure resistance. Ideal for high-recovery purification.
  • Features: Void-free structure for excellent retention and improved integrity, ultra-low protein binding for protein solutions (less than 0.1 mg/mL).
• Ultracel PL Membranes: Regenerated cellulose membranes with optimized permeability for high solute retention and flux levels. They feature a regular non-voided support layer resistant to damage. Best suited for concentration and desalination of solutes, especially with small volumes or low concentrations.
  • Features: Polypropylene backing material for support without impeding flow, low to moderate protein binding.
• VireSolve Membranes: Patented composite membranes designed for reliable and consistent viral clearance. Available in polyvinylidene fluoride (PVDF) or PES variants for normal-flow and tangential-flow filtration.
  • Features: Low-binding structure yields high protein recovery (greater than 90%), provides greater than 3 log removal of viruses.

Operating Limits

Maximum Operating Temperature:

• BioMax PB, Ultracel PLC, Ultracel PL membranes: 80 °C
• Viresolve Membranes (Viresolve 180, Viresolve 180 (Normal Flow Filtration)): 37 °C
• Viresolve Membranes (retrovirus): 80 °C

pH Range:

• BioMax PB membranes: 1–14
• Ultracel PLC membranes: 2–13
• Ultracel PL membranes: 2–10
• Viresolve Membranes: 4–8

Mechanical / Hydraulic

The following parameters are provided for various membrane codes. See the official PDF for exact tabulated values for each specific membrane.

BioMax PB Membranes:

• Membrane Material: Polyethersulfone
• Support Material: Polyolefin Nonwoven
• Water Flux: Ranging from 3.5 lmh/psi to 750 lmh/psi.
• NMWL Cutoff: Ranging from 5 kiloDalton to 1000 kiloDalton, plus 8 kiloDalton for PBSEQ.
• Dextran Retention: 90% at specific kD (e.g., 90% at 10 kD for Biomax 5, 90% at 18 kD for Biomax 8). Some also list BP ≥ 80 psi, BP ≥ 45 psi to ≤ 90 psi, BP ≥ 30 psi to ≤ 55 psi.
• Thickness: 280 microns
• Air Permeability: 0 at 30 psi for most, 1 ml/min at 10 psi for PBVK.
• Max Support Width: 30 inches* or 15 inches** (* 27.5 inches, ** 13.25 inches)
• Standard Length: 400 feet

Ultracel PLC Membranes:

• Membrane Material: Regenerated Cellulose
• Support Material: UHMW Polyethylene
• Average Water Flux: Ranging from 1 lmh/psi to 300 lmh/psi.
• NMWL Cutoff: Ranging from 5 kiloDalton to 1000 kiloDalton.
• Dextran Retention: 90% at specific kD (e.g., 90% at 5 kD for Ultracel 5 kD).
• Average Thickness: 130 microns for most, 200 microns for Ultracel 8 kD.
• Air Permeability: 0 at 70 psi for some, 0 at 30 psi for others, ≤ 2.5 cc/min at 30 psi for Ultracel 300 kD and Ultracel 1000 kD.
• Max Width: 13 inches
• Standard Length: 350 feet

Ultracel PL Membranes:

• Membrane Material: Regenerated Cellulose
• Support Material: Polypropylene Nonwoven
• Water Flux: Ranging from 0.5 lmh/psi to 30 lmh/PSI.
• NMWL Cutoff: Ranging from 1 kiloDalton to 100 kiloDalton.
• Dextran Retention: 90% at specific kD (e.g., 90% at 1 kD for PLAC).
• Thickness: 230 microns
• Air Permeability: < 5 mL/min at 30 psi
• Max Width: 27 inches
• Standard Length: 200 feet

Viresolve Membranes:

• Membrane Material: PVDF or PES (asymmetric)
• Support Material: None
• NMWL Cutoff: 180 kiloDalton (Viresolve 180), NA for retrovirus.
• Thickness: 150 µm (Viresolve 180), 140 µm (retrovirus).
• Water Permeability: 63 lmh/psi (Viresolve 180), 110 lmh/psi (retrovirus).
• Endotoxins: < 0.5 Eu/mL
• Extractables: ≤ 0.3 % (Viresolve 180), < 10 µg C/cm² (retrovirus).
• Virus Retention (LRV): > 1.5 (Viresolve 180), > 6 (retrovirus).
• Max Width: 12 inches (Viresolve 180), 11 inches (retrovirus).
• Standard Length: 500 feet (Viresolve 180), 1,000 feet (retrovirus).

Product Water

Ultrafiltration membranes are used to separate macromolecules, viruses, and colloids from fluids. The primary basis for separation is molecular size.

Nominal Molecular Weight Limit (NMWL) Rating for Concentrating, Desalting, and Buffer Exchange of Macromolecules: The NMWL reflects the molecular weight of solutes retained by the membrane.

• For Macromolecules: Determine the molecular weight (kiloDaltons) of the macromolecule to be retained. For globular proteins, or linear polysaccharides, a chart (not provided here) is used to find the corresponding Stokes radius (nm) and then the recommended Millipore UF membrane.
• For Viruses and Colloids: Determine the radius (nanometers) of the smallest particles. A chart (not provided here) is then used to find the recommended Millipore UF membrane.

Comparative Filtration Technologies

Ultrafiltration is one of several membrane separation techniques, typically handling particles between Reverse Osmosis (RO) and Microfiltration (MF).

• Reverse Osmosis: Filters particles down to 0.2 kDa (e.g., sugars, amino acids, salts).
• Ultrafiltration: Filters particles between 0.2 kDa and 200 kDa (e.g., proteins, polio virus, mammalian virus).
• Microfiltration: Filters particles between 200 kDa and 20,000 kDa (e.g., B. diminuta, yeast, E. coli, bacteria).
• Clarification: Filters larger particles up to 100 µm (e.g., red blood cells, carbon black, pollens, clouds fog, human hair).

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Disclaimer: This summary is based on the provided text extract. It may not include all figures, footnotes, or the most recent revisions of the OEM PDF datasheet. For contractual data or complete specifications, the official OEM PDF revision used on the project should be consulted.

Official datasheet (PDF)

PDF datasheet